Matches in Ubergraph for { <http://purl.obolibrary.org/obo/MI_0112> ?p ?o ?g. }
Showing items 1 to 20 of
20
with 100 items per page.
- MI_0112 IAO_0000115 "The split-ubiquitin system provides a method for examining the interactions of membrane proteins. In the split-ubiquitin system, two integral membrane proteins to be studied are fused to two different ubiquitin moieties: a C-terminal ubiquitin moiety ("Cub", residues 35-76) and an N-terminal ubiquitin moiety ("Nub", residues 1-34). These fused proteins are called the bait and prey, respectively. In addition to being fused to an integral membrane protein, the Cub moiety is also fused to a transcription factor that can be cleaved off by ubiquitin specific proteases. Upon bait-prey interaction, Nub and Cub-moieties assemble, reconstituting the split-ubiquitin. The reconstituted split-ubiquitin molecule is recognized by ubiquitin specific proteases, which cleave off the reporter protein, allowing it to induce the transcription of reporter genes." @default.
- MI_0112 normalizedInformationContent "95.413332725061409" @default.
- MI_0112 referenceCount "2" @default.
- MI_0112 hasExactSynonym "ub reconstruction" @default.
- MI_0112 hasOBONamespace "PSI-MI" @default.
- MI_0112 hasRelatedSynonym "membrane yeast two-hybrid" @default.
- MI_0112 hasRelatedSynonym "myth" @default.
- MI_0112 hasRelatedSynonym "split-ubiquitin" @default.
- MI_0112 id "MI:0112" @default.
- MI_0112 inSubset PSI-MI_slim @default.
- MI_0112 type Class @default.
- MI_0112 isDefinedBy mi.owl @default.
- MI_0112 label "ubiquitin reconstruction" @default.
- MI_0112 subClassOf MI_0000 @default.
- MI_0112 subClassOf MI_0001 @default.
- MI_0112 subClassOf MI_0045 @default.
- MI_0112 subClassOf MI_0090 @default.
- MI_0112 subClassOf MI_0112 @default.
- MI_0112 subClassOf MI_0232 @default.
- MI_0112 subClassOf MI_2412 @default.