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- MI_0113 IAO_0000115 "Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane." @default.
- MI_0113 normalizedInformationContent "90.826665450122817" @default.
- MI_0113 referenceCount "4" @default.
- MI_0113 hasExactSynonym "Immuno blot" @default.
- MI_0113 hasOBONamespace "PSI-MI" @default.
- MI_0113 id "MI:0113" @default.
- MI_0113 inSubset PSI-MI_slim @default.
- MI_0113 type Class @default.
- MI_0113 isDefinedBy mi.owl @default.
- MI_0113 label "western blot" @default.
- MI_0113 subClassOf MI_0000 @default.
- MI_0113 subClassOf MI_0002 @default.
- MI_0113 subClassOf MI_0003 @default.
- MI_0113 subClassOf MI_0113 @default.
- MI_0113 subClassOf MI_0421 @default.
- MI_0113 subClassOf MI_0659 @default.
- MI_0113 subClassOf MI_0661 @default.