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- MI_0226 IAO_0000115 "Stable complexes and their component proteins can be separated on the basis of their net charge by ion-exchange chromatography. If a protein has a net positive charge at pH 7, it will usually bind to a column of beads containing carboxylate groups, and can then be eluted by increasing the concentration of sodium chloride or another salt in the eluting buffer by competition of sodium ions with positively charged groups on the protein for binding to the column. Protein that have a low density of net positive charge will tend to emerge first, followed by those having a higher charge density. Positively charged complexes or proteins (cationic proteins) can be separated on negatively charged carboxymethyl-cellulose (CM-cellulose) columns. Conversely, negatively charged complexes or proteins (anionic proteins) can be separated by chromatography on positively charged diethylaminoethyl-cellulose (DEAE-cellulose) columns." @default.
- MI_0226 normalizedInformationContent "100" @default.
- MI_0226 referenceCount "1" @default.
- MI_0226 hasExactSynonym "IEC" @default.
- MI_0226 hasExactSynonym "ion exchange chrom" @default.
- MI_0226 hasOBONamespace "PSI-MI" @default.
- MI_0226 id "MI:0226" @default.
- MI_0226 inSubset PSI-MI_slim @default.
- MI_0226 type Class @default.
- MI_0226 isDefinedBy mi.owl @default.
- MI_0226 label "ion exchange chromatography" @default.
- MI_0226 subClassOf MI_0000 @default.
- MI_0226 subClassOf MI_0001 @default.
- MI_0226 subClassOf MI_0045 @default.
- MI_0226 subClassOf MI_0091 @default.
- MI_0226 subClassOf MI_0226 @default.
- MI_0226 subClassOf MI_0401 @default.