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- MI_0370 IAO_0000115 "This assay allow identification of interactions in the inner membrane of E. coli. by using a chimeric construct ToxR-TM-MBP composed of the N-terminal DNA binding/transcriptional activation domain of ToxR (a dimerization dependant transcription factor) fused to a transmembrane domain of interest (TM) and a monomeric periplasmic anchor (the maltose binding protein). Association of the two TM results in the ToxR-mediated activation of a reporter gene such as CAT (chloroamphenicol acetyltransferase activity). The level of CAT expression indicates the strength of TM association. CAT expression can then be tested and quantify by measuring CAM resistance with disk diffusion assay or CAT activity assays on cell-free extracts." @default.
- MI_0370 normalizedInformationContent "100" @default.
- MI_0370 referenceCount "1" @default.
- MI_0370 hasExactSynonym "toxcat" @default.
- MI_0370 hasOBONamespace "PSI-MI" @default.
- MI_0370 id "MI:0370" @default.
- MI_0370 inSubset PSI-MI_slim @default.
- MI_0370 type Class @default.
- MI_0370 isDefinedBy mi.owl @default.
- MI_0370 label "tox-r dimerization assay" @default.
- MI_0370 subClassOf MI_0000 @default.
- MI_0370 subClassOf MI_0001 @default.
- MI_0370 subClassOf MI_0045 @default.
- MI_0370 subClassOf MI_0090 @default.
- MI_0370 subClassOf MI_0370 @default.