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- MI_0398 IAO_0000115 "In the pooling strategy sets of either both bait and prey hybrid vectors are mated or, more commonly, individual baits are mated against pools of preys. This approach required cloning baits and preys into both two-hybrid vectors, followed by pooling sets of transformants. The positive double hybrid clones are the interacting partners. The pooling of both baits and prey molecules is now a rarely used technique as the pooling of baits often leads to misleading results." @default.
- MI_0398 normalizedInformationContent "87.123597049860606" @default.
- MI_0398 referenceCount "7" @default.
- MI_0398 hasExactSynonym "two hybrid pooling" @default.
- MI_0398 hasOBONamespace "PSI-MI" @default.
- MI_0398 id "MI:0398" @default.
- MI_0398 inSubset PSI-MI_slim @default.
- MI_0398 type Class @default.
- MI_0398 isDefinedBy mi.owl @default.
- MI_0398 label "two hybrid pooling approach" @default.
- MI_0398 subClassOf MI_0000 @default.
- MI_0398 subClassOf MI_0001 @default.
- MI_0398 subClassOf MI_0018 @default.
- MI_0398 subClassOf MI_0045 @default.
- MI_0398 subClassOf MI_0090 @default.
- MI_0398 subClassOf MI_0232 @default.
- MI_0398 subClassOf MI_0398 @default.