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- MI_0438 IAO_0000115 "In vivo reconstruction of specific RNA-proteins interactions. The DNA binding and transcription activator domains of GAL4 are brought together via the interaction of recombinant RNA. The first hybrid protein contains the DNA binding domain of GAL4 fused to RevM10 (a mutated RNA binding protein of HIV-1 that binds specifically to the Rev responsive element RRE of the env gene). A recombinant RNA contains the RRE sequence and a target RNA sequence X. The second hybrid protein contains the activation domain of GAL4 fused to protein Y tested for its ability to bind the target RNA X. If this interaction occurs the three hybrid reconstructs GAL4 and the transcription of a reporter gene is activated." @default.
- MI_0438 normalizedInformationContent "100" @default.
- MI_0438 referenceCount "1" @default.
- MI_0438 hasExactSynonym "Three hybrid system" @default.
- MI_0438 hasExactSynonym "rna 3-hybrid" @default.
- MI_0438 hasExactSynonym "rna tri hybrid" @default.
- MI_0438 hasExactSynonym "rna-three hybrid" @default.
- MI_0438 hasOBONamespace "PSI-MI" @default.
- MI_0438 id "MI:0438" @default.
- MI_0438 inSubset PSI-MI_slim @default.
- MI_0438 type Class @default.
- MI_0438 isDefinedBy mi.owl @default.
- MI_0438 label "rna three hybrid" @default.
- MI_0438 subClassOf MI_0000 @default.
- MI_0438 subClassOf MI_0001 @default.
- MI_0438 subClassOf MI_0045 @default.
- MI_0438 subClassOf MI_0090 @default.
- MI_0438 subClassOf MI_0232 @default.
- MI_0438 subClassOf MI_0438 @default.
- MI_0438 subClassOf MI_0588 @default.