Matches in Ubergraph for { <http://purl.obolibrary.org/obo/MI_0655> ?p ?o ?g. }
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- MI_0655 IAO_0000115 "A protein of interest (the bait) is fused to the full-length bacteriophage lambda repressor protein (lambdacI, 237 amino acids), containing the amino terminal DNA-binding domain and the carboxylterminal dimerization domain. The corresponding target (prey) protein is fused to the N-terminal domain of the alfa-subunit of RNA polymerase (248 amino acids). The bait is tethered to the lambda operator sequence upstream of the reporter promoter through the DNA-binding domain of lambdacI. When the bait and prey interact, they recruit and stabilize the binding of RNA polymerase at the promoter and activate the transcription of the HIS3 reporter gene. Due to the tendency of both the lambda repressor protein and the N-terminal domain of the alfa-subunit of RNA polymerase to dimerize, this system might not be optimal for the analysis of proteins that self-associate unless their interaction with other protein partners depends on the oligomerization." @default.
- MI_0655 normalizedInformationContent "100" @default.
- MI_0655 referenceCount "1" @default.
- MI_0655 hasExactSynonym "BacterioMatch" @default.
- MI_0655 hasExactSynonym "lambda two hybrid" @default.
- MI_0655 hasOBONamespace "PSI-MI" @default.
- MI_0655 id "MI:0655" @default.
- MI_0655 inSubset PSI-MI_slim @default.
- MI_0655 type Class @default.
- MI_0655 isDefinedBy mi.owl @default.
- MI_0655 label "lambda repressor two hybrid" @default.
- MI_0655 subClassOf MI_0000 @default.
- MI_0655 subClassOf MI_0001 @default.
- MI_0655 subClassOf MI_0045 @default.
- MI_0655 subClassOf MI_0090 @default.
- MI_0655 subClassOf MI_0232 @default.
- MI_0655 subClassOf MI_0655 @default.