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- MI_0728 IAO_0000115 "A chimeric protein consisting of the GAL4 DNA-binding domain (aa 1-147 of GAL4) and a transcriptional activation domain from the herpes simplex virus protein VP16 (either aa 411-490 or aa 411-455) can specifically activate transcription of a reporter gene located downstream ofGAL4 DNA binding sites and the E1B minimal promoter. Similarly, two chimeric proteins, one encoding a chimeric GAL4 protein and the other encoding a chimeric VP16 protein, can activate the reporter gene, if the domains fused to the GAL4 and VP16 sequences can complex with appropriate conformation. However, if the domains fused to the GAL4 and VP16 sequences do not interact specifically to form a + complex that reconstitutes GAL4 function, the reporter gene cannot be activated." @default.
- MI_0728 normalizedInformationContent "100" @default.
- MI_0728 referenceCount "1" @default.
- MI_0728 hasExactSynonym "KISS" @default.
- MI_0728 hasExactSynonym "gal4 vp16 complement" @default.
- MI_0728 hasExactSynonym "karyoplasmic interaction ion strategy" @default.
- MI_0728 hasExactSynonym "mammalian two hybrid" @default.
- MI_0728 hasOBONamespace "PSI-MI" @default.
- MI_0728 id "MI:0728" @default.
- MI_0728 inSubset PSI-MI_slim @default.
- MI_0728 type Class @default.
- MI_0728 isDefinedBy mi.owl @default.
- MI_0728 label "gal4 vp16 complementation" @default.
- MI_0728 subClassOf MI_0000 @default.
- MI_0728 subClassOf MI_0001 @default.
- MI_0728 subClassOf MI_0018 @default.
- MI_0728 subClassOf MI_0045 @default.
- MI_0728 subClassOf MI_0090 @default.
- MI_0728 subClassOf MI_0232 @default.
- MI_0728 subClassOf MI_0728 @default.