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- MI_1197 IAO_0000115 "To perform a radioimmunoassay, a known quantity of an antigen is made radioactive, frequently by labeling it with gamma-radioactive isotopes of iodine attached to tyrosine. This radiolabeled antigen is then mixed with a known amount of antibody for that antigen, and as a result, the two chemically bind to one another. Then, a sample containing an unknown quantity of that same antigen is added. This causes the unlabeled (or "cold") antigen from the serum to compete with the radiolabeled antigen ("hot") for antibody binding sites. As the concentration of "cold" antigen is increased, more of it binds to the antibody, displacing the radiolabeled variant, and reducing the ratio of antibody-bound radiolabeled antigen to free radiolabeled antigen. The bound antigens are then separated from the unbound ones, and the radioactivity of the free antigen remaining in the supernatant is measured." @default.
- MI_1197 normalizedInformationContent "100" @default.
- MI_1197 referenceCount "1" @default.
- MI_1197 created_by "orchard" @default.
- MI_1197 creation_date "2012-06-07T04:13:30Z" @default.
- MI_1197 hasExactSynonym "RIA" @default.
- MI_1197 hasOBONamespace "PSI-MI" @default.
- MI_1197 id "MI:1197" @default.
- MI_1197 type Class @default.
- MI_1197 isDefinedBy mi.owl @default.
- MI_1197 label "radioimmunoassay" @default.
- MI_1197 subClassOf MI_0000 @default.
- MI_1197 subClassOf MI_0002 @default.
- MI_1197 subClassOf MI_0421 @default.
- MI_1197 subClassOf MI_0661 @default.
- MI_1197 subClassOf MI_1197 @default.