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- MI_2171 IAO_0000115 "This technology is based on quantifying the BRET between a pair of participants bearing complementation tags and a participant tagged with a bioluminescent enzyme or a fluorescent tag. The tags held by the pair of participants are complementary halves of either a fluorescent tag (N- and C- fragments of the Venus fluorescent protein, for example) or a bioluminescent enzyme (N- and C- fragments of Renilla luciferase, for example). Interaction between the pair of participants leads to reconstruction of the bioluminescent enzyme/fluorescent protein, which can then emit/accept photons that are accepted/emitted by the tag in the third participant, generating the signal used to detect the interaction. The signals emitted by the bioluminescent enzyme and the fluorescent tag have different wavelengths, so they can be distinguished." @default.
- MI_2171 normalizedInformationContent "100" @default.
- MI_2171 referenceCount "1" @default.
- MI_2171 created_by "ppm" @default.
- MI_2171 creation_date "2015-04-29T13:25:55Z" @default.
- MI_2171 hasExactSynonym "bret complementation assay" @default.
- MI_2171 hasExactSynonym "bret complementation" @default.
- MI_2171 hasExactSynonym "copa-ret" @default.
- MI_2171 hasOBONamespace "PSI-MI" @default.
- MI_2171 id "MI:2171" @default.
- MI_2171 inSubset PSI-MI_slim @default.
- MI_2171 type Class @default.
- MI_2171 isDefinedBy mi.owl @default.
- MI_2171 label "complemented donor-acceptor resonance energy transfer" @default.
- MI_2171 subClassOf MI_0000 @default.
- MI_2171 subClassOf MI_0001 @default.
- MI_2171 subClassOf MI_0013 @default.
- MI_2171 subClassOf MI_0045 @default.
- MI_2171 subClassOf MI_0051 @default.
- MI_2171 subClassOf MI_2169 @default.
- MI_2171 subClassOf MI_2171 @default.