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- MI_2203 IAO_0000115 "Primer extension can be used to determine the 3' end of a given sequence of RNA. This technique requires a radiolabelled primer which is complementary to a region near the 3' end of the RNA. The primer is allowed to anneal to the RNA and reverse transcriptase is used to synthesize a strand of DNA from the RNA until it reaches the 5' end of the RNA. By denaturing the hybrid and using the extended primer DNA strand as a marker on an electrophoretic gel, it is possible to determine the transcriptional start site." @default.
- MI_2203 normalizedInformationContent "100" @default.
- MI_2203 referenceCount "1" @default.
- MI_2203 created_by "ppm" @default.
- MI_2203 creation_date "2015-08-11T17:22:38Z" @default.
- MI_2203 hasExactSynonym "primer extension assay" @default.
- MI_2203 hasOBONamespace "PSI-MI" @default.
- MI_2203 id "MI:2203" @default.
- MI_2203 inSubset PSI-MI_slim @default.
- MI_2203 type Class @default.
- MI_2203 isDefinedBy mi.owl @default.
- MI_2203 label "primer extension assay" @default.
- MI_2203 subClassOf MI_0000 @default.
- MI_2203 subClassOf MI_0002 @default.
- MI_2203 subClassOf MI_0003 @default.
- MI_2203 subClassOf MI_0078 @default.
- MI_2203 subClassOf MI_0659 @default.
- MI_2203 subClassOf MI_0661 @default.
- MI_2203 subClassOf MI_1179 @default.
- MI_2203 subClassOf MI_1193 @default.
- MI_2203 subClassOf MI_2203 @default.