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- MI_2285 IAO_0000115 "The method is based on the functional effect of the binding of the microRNA on the target (mRNA or LncRNA), which is the repression of the expression of the target. To validate a sequence as a direct target of a microRNA, a luciferase reporter gene carrying the wt candidate sequence or its mutated form is used, and the expression of the target is evaluated with a luciferase assay. If the wt is significantly less expressed than the mutant, the binding occurs." @default.
- MI_2285 normalizedInformationContent "100" @default.
- MI_2285 referenceCount "1" @default.
- MI_2285 created_by "pporras" @default.
- MI_2285 creation_date "2018-05-02T15:22:12Z" @default.
- MI_2285 hasExactSynonym "miRNA interference luciferase assay" @default.
- MI_2285 hasOBONamespace "PSI-MI" @default.
- MI_2285 id "MI:2285" @default.
- MI_2285 inSubset PSI-MI_slim @default.
- MI_2285 type Class @default.
- MI_2285 isDefinedBy mi.owl @default.
- MI_2285 label "miRNA interference luciferase reporter assay" @default.
- MI_2285 subClassOf MI_0000 @default.
- MI_2285 subClassOf MI_0001 @default.
- MI_2285 subClassOf MI_0045 @default.
- MI_2285 subClassOf MI_0255 @default.
- MI_2285 subClassOf MI_2285 @default.