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- MI_2288 IAO_0000115 "DAP-seq is an in vitro TF-DNA binding assay in which a DAP-seq gDNA library is prepared by attaching a short DNA sequencing adaptor onto purified and fragmented gDNA. In a separate reaction, an affinity-purified TF is prepared by in vitro expression, bound to ligand-coupled beads, and washed to remove non-specific cellular components. The gDNA library is added to the affinity-bound TF and the unbound DNA is washed away. The bound fraction is eluted, amplified with PCR primers to introduce an indexed adaptor, and the DNA is sequenced." @default.
- MI_2288 normalizedInformationContent "100" @default.
- MI_2288 referenceCount "1" @default.
- MI_2288 created_by "pporras" @default.
- MI_2288 creation_date "2018-06-28T16:09:45Z" @default.
- MI_2288 hasExactSynonym "DNA affinity purification sequencing" @default.
- MI_2288 hasExactSynonym "dap-seq" @default.
- MI_2288 hasOBONamespace "PSI-MI" @default.
- MI_2288 id "MI:2288" @default.
- MI_2288 inSubset PSI-MI_slim @default.
- MI_2288 type Class @default.
- MI_2288 isDefinedBy mi.owl @default.
- MI_2288 label "DAP-seq" @default.
- MI_2288 subClassOf MI_0000 @default.
- MI_2288 subClassOf MI_0001 @default.
- MI_2288 subClassOf MI_0004 @default.
- MI_2288 subClassOf MI_0045 @default.
- MI_2288 subClassOf MI_0091 @default.
- MI_2288 subClassOf MI_0400 @default.
- MI_2288 subClassOf MI_0401 @default.
- MI_2288 subClassOf MI_2288 @default.