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- MI_2361 IAO_0000115 "Bait protein is fused to a standard protein fusion tag and an intein fragment, prey a with a different protein fusion tag and the complementary intein fragment. The bait and prey are co-expressed in a cell line where the association of bait and prey brings the intein fragments into close proximity, allowing them to reconstitute a fully functional intein, which then catalyzes its own excision and the concurrent ligation of the bait and the prey peptides (as well as the standard protein fusion tags). The resulting spliced protein can be resolved by regular western blot analysis due to its altered mobility, while the presence of the standard protein fusion tags allows visualization or purification of protein using regular biochemical techniques." @default.
- MI_2361 normalizedInformationContent "100" @default.
- MI_2361 referenceCount "1" @default.
- MI_2361 created_by "pporras" @default.
- MI_2361 creation_date "2020-12-02T11:31:51Z" @default.
- MI_2361 hasExactSynonym "SIMPL" @default.
- MI_2361 hasExactSynonym "simpl" @default.
- MI_2361 hasOBONamespace "PSI-MI" @default.
- MI_2361 id "MI:2361" @default.
- MI_2361 inSubset PSI-MI_slim @default.
- MI_2361 type Class @default.
- MI_2361 isDefinedBy mi.owl @default.
- MI_2361 label "Split Intein-Mediated Protein Ligation" @default.
- MI_2361 subClassOf MI_0000 @default.
- MI_2361 subClassOf MI_0001 @default.
- MI_2361 subClassOf MI_0045 @default.
- MI_2361 subClassOf MI_0090 @default.
- MI_2361 subClassOf MI_2361 @default.