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- MI_2365 IAO_0000115 "FluoPPI system (Fluorescent Protein-Protein Interaction-visualization):-FLUOPPI utilises the formation of fluorescence foci whereby the interacting proteins of interest are genetically fused with either a tetramerizing fluorescent protein (FP-tag) or an assembly helper tag (Ash-tag). The incorporation of these tags onto a pair of interacting proteins enables the formation of intensely bright foci when co-expressed in mammalian cells. In these foci the FP-tag induces the fused protein to form a tetramer that can now interact with up to 4 copies of the Ash-tagged partner protein. The cognate interacting protein also forms an oligomer through the Ash tag, which in turn can interact with multiple copies of the FP-fused partner protein. The potential of both constructs to interact with multiple copies of each other enable large foci incorporating the fluorescent protein to form, which can be clearly observed when imaging the cell." @default.
- MI_2365 normalizedInformationContent "100" @default.
- MI_2365 referenceCount "1" @default.
- MI_2365 created_by "pporras" @default.
- MI_2365 creation_date "2020-12-07T17:35:40Z" @default.
- MI_2365 hasExactSynonym "FluoPPI" @default.
- MI_2365 hasExactSynonym "fluoppi" @default.
- MI_2365 hasOBONamespace "PSI-MI" @default.
- MI_2365 id "MI:2365" @default.
- MI_2365 type Class @default.
- MI_2365 isDefinedBy mi.owl @default.
- MI_2365 label "fluorescent protein-protein interaction-visualization" @default.
- MI_2365 subClassOf MI_0000 @default.
- MI_2365 subClassOf MI_0001 @default.
- MI_2365 subClassOf MI_0045 @default.
- MI_2365 subClassOf MI_0428 @default.
- MI_2365 subClassOf MI_2365 @default.