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- NCIT_C106050 IAO_0000115 "A molecular genetic technique that combines chromatin immunoprecipitation (ChIP) with DNA microarray technology to find candidate binding sites of DNA-associated proteins in a biological specimen. First, crosslinked protein-DNA complexes are isolated using ChIP. Next, the crosslinks are broken, the proteins are removed and the purified DNA is amplified, denatured and labeled with a fluorescent tag. The labeled, denatured DNA fragments are applied to a DNA microarray where they can hybridize to their complementary DNA sequence. After hybridization occurs, the microarray chip is imaged and the captured fluorescence signals from the array are normalized and subjected to statistical analysis. Finally, validated positive spots on the microarray can predict which DNA sequences interact with the protein of interest." @default.
- NCIT_C106050 NCIT_NHC0 "C106050" @default.
- NCIT_C106050 NCIT_P106 "Molecular Biology Research Technique" @default.
- NCIT_C106050 NCIT_P108 "ChIP-On-Chip" @default.
- NCIT_C106050 NCIT_P207 "C3831112" @default.
- NCIT_C106050 normalizedInformationContent "100" @default.
- NCIT_C106050 referenceCount "1" @default.
- NCIT_C106050 hasExactSynonym "ChIP-Chip" @default.
- NCIT_C106050 hasExactSynonym "ChIP-On-Chip" @default.
- NCIT_C106050 hasExactSynonym "Chromatin Immunoprecipitation-On-Chip" @default.
- NCIT_C106050 type Class @default.
- NCIT_C106050 isDefinedBy ncit.owl @default.
- NCIT_C106050 label "ChIP-On-Chip" @default.
- NCIT_C106050 subClassOf NCIT_C106048 @default.
- NCIT_C106050 subClassOf NCIT_C106050 @default.
- NCIT_C106050 subClassOf NCIT_C116640 @default.
- NCIT_C106050 subClassOf NCIT_C16203 @default.
- NCIT_C106050 subClassOf NCIT_C16623 @default.
- NCIT_C106050 subClassOf NCIT_C16847 @default.
- NCIT_C106050 subClassOf NCIT_C17350 @default.
- NCIT_C106050 subClassOf NCIT_C17632 @default.
- NCIT_C106050 subClassOf NCIT_C19235 @default.
- NCIT_C106050 subClassOf NCIT_C19770 @default.
- NCIT_C106050 subClassOf NCIT_C20368 @default.
- NCIT_C106050 subClassOf NCIT_C25218 @default.
- NCIT_C106050 subClassOf NCIT_C25294 @default.
- NCIT_C106050 subClassOf NCIT_C43431 @default.