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- NCIT_C54164 IAO_0000115 "Recognizing that only a single tryptic peptide is needed to quantify the expression of the corresponding parent protein, the ICAT reagent was designed to affinity isolate and quantify via the use of a stable isotope the relative concentrations of cysteine-containing tryptic peptides obtained from digests of control versus experimental samples. A feature of the ICAT approach is that the in vitro incorporation of a stable isotope into one of the two samples being compared obviates the need to analyze by mass spectrometry the control and experimental samples separately. ICAT data, which is analogous to that obtained via the use of two different fluorescent dyes in DNA microarray analysis of mRNA or DIGE analysis of protein expression, provides the corresponding ratio for the level of expression of the parent protein in the control versus experimental sample." @default.
- NCIT_C54164 NCIT_NHC0 "C54164" @default.
- NCIT_C54164 NCIT_P106 "Research Activity" @default.
- NCIT_C54164 NCIT_P108 "Isotope-Coded Affinity Tag Protein Analysis" @default.
- NCIT_C54164 NCIT_P207 "C1708579" @default.
- NCIT_C54164 NCIT_P366 "Isotope-Coded_Affinity_Tag_Protein_Analysis" @default.
- NCIT_C54164 normalizedInformationContent "100" @default.
- NCIT_C54164 referenceCount "1" @default.
- NCIT_C54164 hasExactSynonym "ICAT Analysis" @default.
- NCIT_C54164 hasExactSynonym "ICAT Profiling" @default.
- NCIT_C54164 hasExactSynonym "ICAT" @default.
- NCIT_C54164 hasExactSynonym "Isotope-Coded Affinity Tag Analysis" @default.
- NCIT_C54164 hasExactSynonym "Isotope-Coded Affinity Tag Protein Analysis" @default.
- NCIT_C54164 type Class @default.
- NCIT_C54164 isDefinedBy ncit.owl @default.
- NCIT_C54164 label "Isotope-Coded Affinity Tag Protein Analysis" @default.
- NCIT_C54164 subClassOf NCIT_C16847 @default.
- NCIT_C54164 subClassOf NCIT_C19236 @default.
- NCIT_C54164 subClassOf NCIT_C20368 @default.
- NCIT_C54164 subClassOf NCIT_C43431 @default.
- NCIT_C54164 subClassOf NCIT_C54164 @default.