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- B01f128b722bee2e28c2e94f3608877e0 hasDbXref "PMID:7708014" @default.
- B01f128b722bee2e28c2e94f3608877e0 type Axiom @default.
- B01f128b722bee2e28c2e94f3608877e0 annotatedProperty IAO_0000115 @default.
- B01f128b722bee2e28c2e94f3608877e0 annotatedSource MI_0049 @default.
- B01f128b722bee2e28c2e94f3608877e0 annotatedTarget "A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on the nitrocellulose filters. The method is highly general and therefore widely applicable. A variety of approaches can be used to label the ligand, alternatively the ligand can be detected by a specific antibody." @default.