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- B25579f447719dcbbfcfd84e9eb040577 hasDbXref "PMID:11827829" @default.
- B25579f447719dcbbfcfd84e9eb040577 type Axiom @default.
- B25579f447719dcbbfcfd84e9eb040577 annotatedProperty IAO_0000115 @default.
- B25579f447719dcbbfcfd84e9eb040577 annotatedSource MI_0095 @default.
- B25579f447719dcbbfcfd84e9eb040577 annotatedTarget "ProteinChip(r) Array technology is a surface-enhanced laser desorption/ionization (SELDI) approach (Ciphergen Biosystems Inc. Fremont, CA, USA) for sample fractionation accomplished by retentate chromatography. Retentate chromatography is performed on ProteinChip Arrays with varying chromatographic properties (e.g. anion exchange, cation exchange, metal affinity and reverse phase). By utilising arrays with differing surface chemistries in parallel and in series, a complex mixture of proteins, as from cells or body fluids, can be resolved into subsets of proteins with common properties. Specific analytes can also be examined by using preactivated arrays to which a bait molecule (such as an antibody or biotinylated DNA) is immobilized and a solution containing the binding partner(s) is presented to the array. This array-based immunoprecipitation or protein-binding experiment has been used with good success to study DNA-binding proteins, receptor-ligand interactions, and protein complexes. Any ligand retained on a SELDI chip can directly be identified by mass spectrometry." @default.