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- B375fed4edf4f6f083729c9f078160190 hasDbXref "PMID:14755292" @default.
- B375fed4edf4f6f083729c9f078160190 type Axiom @default.
- B375fed4edf4f6f083729c9f078160190 annotatedProperty IAO_0000115 @default.
- B375fed4edf4f6f083729c9f078160190 annotatedSource MI_0021 @default.
- B375fed4edf4f6f083729c9f078160190 annotatedTarget "Two proteins can be localised to cell compartments, in the same experiment, if they are expressed as chimeric proteins fused to distinct proteins fluorescing at different wavelengths (Green Fluorescent Protein and Red Fluorescent Protein for example). Using a confocal microscope the two proteins can be visualized in living cells and it can be determined whether they have the same subcellular location. Fluorescence microscopy of cells expressing a GFP fusion protein can also demonstrate dynamic processes such as its translocation from one subcellular compartment to another. OBSOLETE: use imaging technique (MI:0428) and specific probe as feature of each interacting protein." @default.