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- B5c556d074d7de3219dc4c96db7449582 hasDbXref "PMID:14755292" @default.
- B5c556d074d7de3219dc4c96db7449582 type Axiom @default.
- B5c556d074d7de3219dc4c96db7449582 annotatedProperty IAO_0000115 @default.
- B5c556d074d7de3219dc4c96db7449582 annotatedSource MI_0113 @default.
- B5c556d074d7de3219dc4c96db7449582 annotatedTarget "Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane." @default.