Matches in Ubergraph for { <https://frink.apps.renci.org/.well-known/genid/B6f734bcbcb5ba755946c3f54debaf55f> ?p ?o ?g. }
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- B6f734bcbcb5ba755946c3f54debaf55f hasDbXref "PMID:11551470" @default.
- B6f734bcbcb5ba755946c3f54debaf55f hasDbXref "PMID:12167034" @default.
- B6f734bcbcb5ba755946c3f54debaf55f type Axiom @default.
- B6f734bcbcb5ba755946c3f54debaf55f annotatedProperty IAO_0000115 @default.
- B6f734bcbcb5ba755946c3f54debaf55f annotatedSource MI_0098 @default.
- B6f734bcbcb5ba755946c3f54debaf55f annotatedTarget "This method permits the coupling of phenotype to genotype via the formation of a non-covalent ternary complex between mRNAs and their encoded polypeptides while they are translated in an in vitro system. As a first step a cDNA library is constructed that encodes chimeric proteins in which the natural proteins or protein domains are fused to a C-terminal tether. As a consequence when the mRNA is translated in vitro the domain can fold while the tether is still in the ribosomal tunnel. Furthermore this chimeric mRNAs lack a stop codon, thus preventing release of the mRNA and the polypeptide from the ribosome. High concentrations of magnesium and low temperature further stabilise the ternary complex. Similarly to phage display, these complexes can be used directly to select for nucleic acids encoding proteins with desired properties." @default.