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- B85deb250d8c8d8d40a9b899a7fe7d381 NCIT_P378 "NCI" @default.
- B85deb250d8c8d8d40a9b899a7fe7d381 type Axiom @default.
- B85deb250d8c8d8d40a9b899a7fe7d381 annotatedProperty IAO_0000115 @default.
- B85deb250d8c8d8d40a9b899a7fe7d381 annotatedSource NCIT_C106054 @default.
- B85deb250d8c8d8d40a9b899a7fe7d381 annotatedTarget "A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in a DNA sample. First, a denatured DNA sample is treated with bisulfite which converts non-methylated cytosine to uracil. Next, the sample is amplified using a PCR method that does not discriminate between non-methylated and methylated sequences. The amplified DNA is subjected to nucleotide sequencing. The resulting sequence is compared to an identical control sample of DNA that was not treated with bisulfite. Unmethylated cytosines will be displayed as cytosines in the control sample and as thymines in the bisulfite-treated sample." @default.