Matches in Ubergraph for { <https://frink.apps.renci.org/.well-known/genid/B8db87bc818463eb6557d2fbbdde45d92> ?p ?o ?g. }
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- B8db87bc818463eb6557d2fbbdde45d92 hasDbXref "PMID:15792953" @default.
- B8db87bc818463eb6557d2fbbdde45d92 type Axiom @default.
- B8db87bc818463eb6557d2fbbdde45d92 annotatedProperty IAO_0000115 @default.
- B8db87bc818463eb6557d2fbbdde45d92 annotatedSource MI_0655 @default.
- B8db87bc818463eb6557d2fbbdde45d92 annotatedTarget "A protein of interest (the bait) is fused to the full-length bacteriophage lambda repressor protein (lambdacI, 237 amino acids), containing the amino terminal DNA-binding domain and the carboxylterminal dimerization domain. The corresponding target (prey) protein is fused to the N-terminal domain of the alfa-subunit of RNA polymerase (248 amino acids). The bait is tethered to the lambda operator sequence upstream of the reporter promoter through the DNA-binding domain of lambdacI. When the bait and prey interact, they recruit and stabilize the binding of RNA polymerase at the promoter and activate the transcription of the HIS3 reporter gene. Due to the tendency of both the lambda repressor protein and the N-terminal domain of the alfa-subunit of RNA polymerase to dimerize, this system might not be optimal for the analysis of proteins that self-associate unless their interaction with other protein partners depends on the oligomerization." @default.