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- Be77603961be7d2cddd82b7261d5ea77c hasDbXref "PMID:11160938" @default.
- Be77603961be7d2cddd82b7261d5ea77c type Axiom @default.
- Be77603961be7d2cddd82b7261d5ea77c annotatedProperty IAO_0000115 @default.
- Be77603961be7d2cddd82b7261d5ea77c annotatedSource MI_0097 @default.
- Be77603961be7d2cddd82b7261d5ea77c annotatedTarget "In this complementation approach the bait can be any membrane protein (for example a receptor or a channel protein), the prey is cloned as a fusion protein of any cDNA from a library and the coding sequence of cytoplasmic RAS (cdc25 in yeast). If the bait and the prey interact, RAS is recruited close to the membrane and can activate cell growth. This procedure must take place in cells having a mutated RAS (Cdc25-2 yeast strain having a temperature sensitive mutation of RAS) to avoid constitutive growth activation." @default.