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- Bf0af7f6c2393ce34ff1d5d00718cfe7d NCIT_P378 "NCI" @default.
- Bf0af7f6c2393ce34ff1d5d00718cfe7d type Axiom @default.
- Bf0af7f6c2393ce34ff1d5d00718cfe7d annotatedProperty IAO_0000115 @default.
- Bf0af7f6c2393ce34ff1d5d00718cfe7d annotatedSource NCIT_C106054 @default.
- Bf0af7f6c2393ce34ff1d5d00718cfe7d annotatedTarget "A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in a DNA sample. First, a denatured DNA sample is treated with bisulfite which converts non-methylated cytosine to uracil. Next, the sample is amplified using a PCR method that does not discriminate between non-methylated and methylated sequences. The amplified DNA is subjected to nucleotide sequencing. The resulting sequence is compared to an identical control sample of DNA that was not treated with bisulfite. Unmethylated cytosines will be displayed as cytosines in the control sample and as thymines in the bisulfite-treated sample." @default.