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- W100055952 abstract "This chapter reviews the characterization of human natural killer (NK) cells identified by the monoclonal HNK-1 (Leu-7) antibody. The monoclonal antibody, HNK-1, was produced against the cultured cell line, HSB-2, using the hybridoma technique introduced by Köhler and Milstein. Although HSB-2 cells have been considered to be of T-cell origin, they lack T-cell-associated antigens identified by the monoclonal antibodies, OKT1, T3, T4, T5, and T8. In a study described in the chapter, when the HNK-1 antibody was tested against cultured lymphoid cell lines, it reacted with some T-cell lines (HSB-2 and MOLT-4) but not with any other T-cell line (MOLT-3), with any B-cell lines, or with cultured phagocytic cells. When human blood mononuclear cells were examined by immunofluorescence, the HNK-1 antibody reacted solely with a morphologically distinct population of granular lymphocytes with NK- and K-cell function. The results suggested that a differentiation antigen identified by the monoclonal HNK-1 antibody is exclusively expressed on human NK and K cells. The HNK-1 antibody also distinguishes the classically defined NK cell from its analogues." @default.
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- W100055952 date "1982-01-01" @default.
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- W100055952 title "CHARACTERIZATION OF HUMAN NK CELLS IDENTIFIED BY THE MONOCLONAL HNK-1 (Leu-7) ANTIBODY" @default.
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- W100055952 doi "https://doi.org/10.1016/b978-0-12-341360-4.50011-1" @default.
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