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- W1001072904 abstract "Publisher Summary This chapter discusses the culture of mammalian retinal pigment epithelium and neural retina. Its purpose is to describe methods of preparing primary monolayer cultures of mammalian retinal pigment epithelium (PE) and neural retina. The method illustrates removing eyes from 6 to 8 day-old pigmented rats and allows them to soak overnight in calcium-enriched Puck's. Incubate the eyes in an enzyme mixture for 45 min at 37°. Under a dissecting microscope, the anterior segment and the neural retina are removed and the intact sheets of PE are teased from each eyecup. The method is equally applicable to normal pigmented rats and to pigmented Royal College of Surgeons (RCS) rats with hereditary retinal dystrophy. With practice the method can also be used to isolate and culture PE from albino rats. Cultured rat PE has been used to study the phagocytosis of rod outer segments 15.26,27 and taurine uptake. The use of pigmented rats greatly facilitates the detection of pigment epithelial contamination." @default.
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- W1001072904 title "[6] Culture of mammalian retinal pigment epithelium and neural retina" @default.
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- W1001072904 doi "https://doi.org/10.1016/s0076-6879(82)81008-2" @default.
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