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- W100114580 abstract "Neuroparsins (NPs) are two distinct and differently charged proteins, present in 70% methanolic extracts of nervous corpora cardiaca (N-CC) of Locusta migratoria. Since their production in the central nervous system is restricted to the Al type of neurosecretory cells from the pars intercerebralis (PI), they were named neuroparsins (Girardie et al., 1987a; Bourême et al., 1987). Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the two NPs had the same apparent molecular weight. Reduction by 2 mercaptoethanol demonstrated that both NPs were constituted of two polypeptide chains of the same apparent molecular weight linked by disulfide bridges (Girardie et al., 1985). In vivo labelling with 35S cysteine revealed that only the most basic NP was present in the PI while both NPs were stored in the N-CC (Girardie et al., 1987b). In vitro enzymatic transformation of the PI-NP into NP stored only in the N-CC was obtained following incubation of PI-NP in crude extracts of N-CC (Bourême and Girardie, 1988). Consequently, we designated the parent NP as NPA and the mature NP as NPB." @default.
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- W100114580 date "1990-01-01" @default.
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- W100114580 title "Sequencing and Biological Activities of Neuroparsins Isolated from Corpora Cardiaca of Locusta Migratoria" @default.
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- W100114580 doi "https://doi.org/10.1007/978-1-4612-4512-4_10" @default.
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