Matches in SemOpenAlex for { <https://semopenalex.org/work/W1001447167> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W1001447167 endingPage "2388" @default.
- W1001447167 startingPage "2386" @default.
- W1001447167 abstract "Objective To isolate the influenza A (H1N1) virus in vitro, and to explore the biological characteristics of the hemagglutinin gene. Methods Nasopharyngeal secretion was collected from the influenza virus sufferers and viral isolation was carried out by MDCK cells. The total RNA was extracted. One step RT-PCR was utilized to amplify the ORF fragment of HA gene with specific primers, and then the product was cloned to pUCm-T vector and pUCm-T/HA was transferred to Escherichia coli XL1-Blue. After the positive clone was identified by blue-white selection assay and PCR, the sequencing was analyzed by ABI sequencer 3730 and the structure characteristics were analyzed by Blast, DNAStar or BioEdit software. Results The influenza A virus was successfully isolated through MDCK cells culture and the cells appeared to CPE after 24 hours, the second generation of the virus' titer was 256 by hemagglutination inhibition assay (HAI). The PCR product was about 1, 701 bp and the pUCm-T/HA vector was successfully constructed. The homology of nucleotide and amino acid was 99% similar to the strain of A/California/04/2009 (H1N1), and the isolated strain contained 566 amino acid which composed by 20 types of amino acid. Conclusions The successful isolation and gene sequence analysis will be of great benefit to the later research of HA protein." @default.
- W1001447167 created "2016-06-24" @default.
- W1001447167 creator A5002219937 @default.
- W1001447167 creator A5038266100 @default.
- W1001447167 creator A5074410974 @default.
- W1001447167 date "2010-12-25" @default.
- W1001447167 modified "2023-09-27" @default.
- W1001447167 title "人源性甲型流感病毒(H1N1)分离株血凝素生物学特征分析" @default.
- W1001447167 hasPublicationYear "2010" @default.
- W1001447167 type Work @default.
- W1001447167 sameAs 1001447167 @default.
- W1001447167 citedByCount "0" @default.
- W1001447167 crossrefType "journal-article" @default.
- W1001447167 hasAuthorship W1001447167A5002219937 @default.
- W1001447167 hasAuthorship W1001447167A5038266100 @default.
- W1001447167 hasAuthorship W1001447167A5074410974 @default.
- W1001447167 hasConcept C104317684 @default.
- W1001447167 hasConcept C153911025 @default.
- W1001447167 hasConcept C159047783 @default.
- W1001447167 hasConcept C182072434 @default.
- W1001447167 hasConcept C2522874641 @default.
- W1001447167 hasConcept C2778692840 @default.
- W1001447167 hasConcept C32611913 @default.
- W1001447167 hasConcept C34343649 @default.
- W1001447167 hasConcept C54355233 @default.
- W1001447167 hasConcept C61053724 @default.
- W1001447167 hasConcept C81089528 @default.
- W1001447167 hasConcept C86803240 @default.
- W1001447167 hasConcept C89423630 @default.
- W1001447167 hasConceptScore W1001447167C104317684 @default.
- W1001447167 hasConceptScore W1001447167C153911025 @default.
- W1001447167 hasConceptScore W1001447167C159047783 @default.
- W1001447167 hasConceptScore W1001447167C182072434 @default.
- W1001447167 hasConceptScore W1001447167C2522874641 @default.
- W1001447167 hasConceptScore W1001447167C2778692840 @default.
- W1001447167 hasConceptScore W1001447167C32611913 @default.
- W1001447167 hasConceptScore W1001447167C34343649 @default.
- W1001447167 hasConceptScore W1001447167C54355233 @default.
- W1001447167 hasConceptScore W1001447167C61053724 @default.
- W1001447167 hasConceptScore W1001447167C81089528 @default.
- W1001447167 hasConceptScore W1001447167C86803240 @default.
- W1001447167 hasConceptScore W1001447167C89423630 @default.
- W1001447167 hasIssue "12" @default.
- W1001447167 hasLocation W10014471671 @default.
- W1001447167 hasOpenAccess W1001447167 @default.
- W1001447167 hasPrimaryLocation W10014471671 @default.
- W1001447167 hasVolume "17" @default.
- W1001447167 isParatext "false" @default.
- W1001447167 isRetracted "false" @default.
- W1001447167 magId "1001447167" @default.
- W1001447167 workType "article" @default.