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- W1001528983 abstract "A dot-immunobinding assay (DIA) was evaluated for detecting Pseu domonas syringae pv. syringae, P. s. pv. atrofaciens, Clavibacter tritici, and Xanthomonas campestris pv. translucens in wheat seeds. Cross reactions between antigens and antisera of P. s. pv. syringae and P. s. pv. atrofaciens negated the use of DIA in distinguishing these incitants. X. c. pv. translucens provided positive readings only when cell concentrations were 105 colony forming units (CFU/ml) or higher, tritici cells were also detectable at 105 CFU/ml. The DIA was most valuable in identifying X. c. pv. translucens colonies after the seed leachate was plated on semi selective media. The assay was not reliable for detecting low cell numbers directly from seed. Cell concentrations of X. c. pv. translucens declined only about 10-fold in wheat seeds after three years of storage. Additional Index Words: Basal glume rot, black chaff, spike blight, bacterial leaf blight." @default.
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- W1001528983 date "1987-01-01" @default.
- W1001528983 modified "2023-09-27" @default.
- W1001528983 title "Evaluation of the dot-immunobinding assay for detecting phytopathogenic bateria in wheat seeds." @default.
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