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- W1004042023 abstract "Publisher Summary Three DNA polymerases have been isolated from Bacillus subtilis. The first of these, polymerase I, was purified in 1964 by Okazaki and Kornberg. Recently, mutants lacking polymerase I (polA — ) have been identified. The investigations into the residual polymerase activity in polA — ceils have resulted in the isolation of two other DNA polymerases called polymerase II and polymerase III, in order of discovery. The three enzymes bear some similarities to the correspondingly numbered DNA polymerases from Escherichia coli . The three enzymes catalyze the covalent addition of nucleotides to a DNA primer under the direction of a DNA template using the four common deoxyribonucleoside 5'-triphosphates as substrate. The routine assay measures the incorporation of a radioactively labeled nucleoside triphosphate into a form that is insoluble in acid." @default.
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- W1004042023 date "1974-01-01" @default.
- W1004042023 modified "2023-10-17" @default.
- W1004042023 title "[4] Bacillus subtilis DNA polymerases" @default.
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- W1004042023 doi "https://doi.org/10.1016/0076-6879(74)29006-2" @default.
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