Matches in SemOpenAlex for { <https://semopenalex.org/work/W1004847654> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W1004847654 endingPage "529" @default.
- W1004847654 startingPage "522" @default.
- W1004847654 abstract "Publisher Summary This chapter discusses enzymatic synthesis of polyguanylic acid and copolymers containing guanylic acid. The synthesis of high molecular weight polyguanylie acid proceed with great difficulty under conditions where the other homopolymers are readily formed. Polyguanylic acid can be obtained without a primer when the concentration of substrate GDP and Mg ++ are very low but in the presence of a large amount of enzyme. Thus, with 1 m M GDP and polynucleotide phosphorylase extracted from Azotobacter vinelandii, high molecular weight poly G is formed. When polymerization of GDP, catalyzed by highly purified polynucleotide phosphorylase isolated from Escherichia coli , is made at 60° and in the presence of Mn ++ instead of Mg ++ , the reaction proceeds readily; the procedure is quite reproducible. The polyguanylic acid thus obtained is fairly homogeneous and has a high molecular weight. The only limiting factor is the enzyme. Polynucleotide phosphorylase isolated from Escherichia coli and purified to at least DEAE-Sephadex step can synthesize the polyguanylic acid under the conditions described in the chapter, as far as the yield and the properties of the polymer are concerned. At a lower temperature, polynucleotide phosphorylase isolated from Micrococcus lysodeikticus can also synthesize poly G in the presence of a primer. Enzymatic synthesis of copolymers in the presence of high ratio of GDP also presents some difficulty whether in the presence or the absence of primer. The ratio of guanylic acid to other nucleotides incorporated into the copolymers is always higher than the ratio of GDP to other nucleoside diphosphatcs in the input pool." @default.
- W1004847654 created "2016-06-24" @default.
- W1004847654 creator A5057348062 @default.
- W1004847654 creator A5087131734 @default.
- W1004847654 date "1968-01-01" @default.
- W1004847654 modified "2023-09-23" @default.
- W1004847654 title "[136] Enzymatic synthesis of polyguanylic acid and copolymers containing guanylic acid" @default.
- W1004847654 cites W1544018772 @default.
- W1004847654 cites W159687900 @default.
- W1004847654 cites W1966071984 @default.
- W1004847654 cites W1976667064 @default.
- W1004847654 cites W1984493196 @default.
- W1004847654 cites W2037658028 @default.
- W1004847654 cites W2051291774 @default.
- W1004847654 cites W2085870396 @default.
- W1004847654 cites W2102279369 @default.
- W1004847654 cites W2103865815 @default.
- W1004847654 cites W2276969945 @default.
- W1004847654 cites W3024096007 @default.
- W1004847654 cites W3118683452 @default.
- W1004847654 cites W4231106700 @default.
- W1004847654 cites W4246236663 @default.
- W1004847654 cites W51261522 @default.
- W1004847654 doi "https://doi.org/10.1016/0076-6879(67)12163-0" @default.
- W1004847654 hasPublicationYear "1968" @default.
- W1004847654 type Work @default.
- W1004847654 sameAs 1004847654 @default.
- W1004847654 citedByCount "17" @default.
- W1004847654 crossrefType "book-chapter" @default.
- W1004847654 hasAuthorship W1004847654A5057348062 @default.
- W1004847654 hasAuthorship W1004847654A5087131734 @default.
- W1004847654 hasConcept C15920480 @default.
- W1004847654 hasConcept C178790620 @default.
- W1004847654 hasConcept C181199279 @default.
- W1004847654 hasConcept C185592680 @default.
- W1004847654 hasConcept C521977710 @default.
- W1004847654 hasConcept C55493867 @default.
- W1004847654 hasConceptScore W1004847654C15920480 @default.
- W1004847654 hasConceptScore W1004847654C178790620 @default.
- W1004847654 hasConceptScore W1004847654C181199279 @default.
- W1004847654 hasConceptScore W1004847654C185592680 @default.
- W1004847654 hasConceptScore W1004847654C521977710 @default.
- W1004847654 hasConceptScore W1004847654C55493867 @default.
- W1004847654 hasLocation W10048476541 @default.
- W1004847654 hasOpenAccess W1004847654 @default.
- W1004847654 hasPrimaryLocation W10048476541 @default.
- W1004847654 hasRelatedWork W1164537646 @default.
- W1004847654 hasRelatedWork W1850986032 @default.
- W1004847654 hasRelatedWork W1868923609 @default.
- W1004847654 hasRelatedWork W1968721630 @default.
- W1004847654 hasRelatedWork W2019529003 @default.
- W1004847654 hasRelatedWork W2022420750 @default.
- W1004847654 hasRelatedWork W2084240653 @default.
- W1004847654 hasRelatedWork W2115477213 @default.
- W1004847654 hasRelatedWork W3104068210 @default.
- W1004847654 hasRelatedWork W4242974861 @default.
- W1004847654 isParatext "false" @default.
- W1004847654 isRetracted "false" @default.
- W1004847654 magId "1004847654" @default.
- W1004847654 workType "book-chapter" @default.