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- W1005953758 abstract "This chapter discusses the preparation of higher plant chloroplasts, concentrates on the possible sources of difficulty in separation of intact chloroplasts from spinach, and mentions of some relevant matters that have become clearer in the recent past. Fully functional chloroplasts may be readily separated from spinach protoplasts. Chloroplasts isolated from young pea shoots have different permeability characteristics to those from mature spinach. For culture preparation in the case of peas, seeds are soaked overnight in aerated water and germinated, in vermiculite, in flat trays at a density of 125 gm (dry weight) per tray (38 × 23 × 8 cm). The combination of low light and high temperature is avoided, but in relatively strong light (>50 W/m 2) temperatures of 20–25° and photoperiods of 9–12 hours, good material can be grown in 9–11 days, by that time, the shoots of a variety are 3–4 cm high. For spinach, any one of a number of sugars or sugar alcohols may be used as osmotica. Good chloroplasts can be prepared in the absence of Mg, but the presence of Mg together with EDTA appears to be beneficial." @default.
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- W1005953758 date "1980-01-01" @default.
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- W1005953758 title "[9] Preparation of higher plant chloroplasts" @default.
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- W1005953758 doi "https://doi.org/10.1016/s0076-6879(80)69011-9" @default.
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