Matches in SemOpenAlex for { <https://semopenalex.org/work/W100803621> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W100803621 abstract "We report a methodology suitable for the large scale production and partial purification of human lymphoblastoid interferon with a minimum expense and reasonable (10%) degree of purity of product. The cells used were Namalwa lymphoblastoid cells, which have the advantage of being both easy to grow and well characterized. They were grown in RPMI 1640 containing 10% fetal calf serum to a density of 1.5 - 2 X 10(6) cells/ml and then diluted 50% by the addition of serum free medium containing 2mM sodium butyrate. After 48 hours, the medium was removed and the cells induced to produce interferon by Sendai virus. Typical initial interferon titres were in the region of 4 - 4.8 log units/ml, while specific activities were in the region of 10(6) units/mg protein. The initial stage in the purification involved batchwise adsorption of the interferon to Procion red- HE7B Sepharose CL6B . The Sepharose was then packed into a column, washed with 0.5M KC1 and the interferon eluted with 2M KC1. The interferon was further purified by gel filtration to give an activity of approximately 10(7) units/mg protein. Yields were between 30-50% of the initial interferon in a volume of 25 ml. Further separation of the components of the heterogeneous alpha interferon could be obtained on Procion blue-HER Sepharose, or by utilizing reverse phase HPLC." @default.
- W100803621 created "2016-06-24" @default.
- W100803621 creator A5064387012 @default.
- W100803621 creator A5086327516 @default.
- W100803621 date "1984-01-01" @default.
- W100803621 modified "2023-09-27" @default.
- W100803621 title "A Simple Methodology for the Routine Production and Partial Purification of Human Lymphoblastoid Interferon" @default.
- W100803621 cites W1995631340 @default.
- W100803621 cites W2003004828 @default.
- W100803621 cites W2014321075 @default.
- W100803621 cites W2063745155 @default.
- W100803621 cites W2076427033 @default.
- W100803621 cites W2079309623 @default.
- W100803621 cites W2088142386 @default.
- W100803621 cites W2100837269 @default.
- W100803621 cites W2147598378 @default.
- W100803621 cites W2169285765 @default.
- W100803621 cites W4243754378 @default.
- W100803621 doi "https://doi.org/10.1007/978-1-4615-9376-8_16" @default.
- W100803621 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6328906" @default.
- W100803621 hasPublicationYear "1984" @default.
- W100803621 type Work @default.
- W100803621 sameAs 100803621 @default.
- W100803621 citedByCount "0" @default.
- W100803621 crossrefType "book-chapter" @default.
- W100803621 hasAuthorship W100803621A5064387012 @default.
- W100803621 hasAuthorship W100803621A5086327516 @default.
- W100803621 hasConcept C104317684 @default.
- W100803621 hasConcept C113842279 @default.
- W100803621 hasConcept C159047783 @default.
- W100803621 hasConcept C181199279 @default.
- W100803621 hasConcept C185592680 @default.
- W100803621 hasConcept C2776178377 @default.
- W100803621 hasConcept C2779881004 @default.
- W100803621 hasConcept C2780127873 @default.
- W100803621 hasConcept C43617362 @default.
- W100803621 hasConcept C54355233 @default.
- W100803621 hasConcept C55493867 @default.
- W100803621 hasConcept C73826308 @default.
- W100803621 hasConcept C81885089 @default.
- W100803621 hasConcept C86803240 @default.
- W100803621 hasConceptScore W100803621C104317684 @default.
- W100803621 hasConceptScore W100803621C113842279 @default.
- W100803621 hasConceptScore W100803621C159047783 @default.
- W100803621 hasConceptScore W100803621C181199279 @default.
- W100803621 hasConceptScore W100803621C185592680 @default.
- W100803621 hasConceptScore W100803621C2776178377 @default.
- W100803621 hasConceptScore W100803621C2779881004 @default.
- W100803621 hasConceptScore W100803621C2780127873 @default.
- W100803621 hasConceptScore W100803621C43617362 @default.
- W100803621 hasConceptScore W100803621C54355233 @default.
- W100803621 hasConceptScore W100803621C55493867 @default.
- W100803621 hasConceptScore W100803621C73826308 @default.
- W100803621 hasConceptScore W100803621C81885089 @default.
- W100803621 hasConceptScore W100803621C86803240 @default.
- W100803621 hasLocation W1008036211 @default.
- W100803621 hasLocation W1008036212 @default.
- W100803621 hasOpenAccess W100803621 @default.
- W100803621 hasPrimaryLocation W1008036211 @default.
- W100803621 hasRelatedWork W100803621 @default.
- W100803621 hasRelatedWork W1521350737 @default.
- W100803621 hasRelatedWork W1797943713 @default.
- W100803621 hasRelatedWork W2004105442 @default.
- W100803621 hasRelatedWork W2053774504 @default.
- W100803621 hasRelatedWork W2088913921 @default.
- W100803621 hasRelatedWork W2117735867 @default.
- W100803621 hasRelatedWork W2161574373 @default.
- W100803621 hasRelatedWork W2418371300 @default.
- W100803621 hasRelatedWork W2462463688 @default.
- W100803621 isParatext "false" @default.
- W100803621 isRetracted "false" @default.
- W100803621 magId "100803621" @default.
- W100803621 workType "book-chapter" @default.