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- W1008040401 abstract "Publisher Summary This chapter focuses on the quantifying thrombin-catalyzed release of fibrinopeptides from fibrinogen using high-performance liquid chromatography (HPLC). It presents several methods in which the HPLC analysis of fibrinopeptides can yield (1) initial rates of thrombin-catalyzed release of fibrinopeptides from fibrinogen, (2) the Miehaelis–Menten parameters catalytic constant( k CAT ) and (Michaelis constant) K M for a-thrombin-catalyzed release of fibrinopeptide A (FPA) and fibilnopeptide B (FPB) from fibrinogen and fibrin I, (3) the specificity constant k CAT / K M for both FPA and FPB at concentrations of fibrinogen below K M , and (4) the inhibition constant for hirugen, an exosite inhibitor of α-thrombin. The chapter also discusses the HPLC profile of coeluting fibrinopeptides and activation peptides from factor XIII. The chapter describes a method for determining the concentration of α-thrombin that is catalytically competent to process fibrinogen." @default.
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- W1008040401 date "1993-01-01" @default.
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- W1008040401 title "[20] Quantifying thrombin-catalyzed release of fibrinopeptides from fibrinogen using high-performance liquid chromatography" @default.
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- W1008040401 doi "https://doi.org/10.1016/0076-6879(93)22023-9" @default.
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