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- W1008065051 abstract "The pET vectors are the mode of expression in prokaryotic system.The pET22b(+) and Escherichia coli BL21(DE3) were used as expression vector and host strain,respectively.The secretory condition of mannanase was optimized by considering several parameters,including media,temperature,IPTG concentration,time of induction,and induction at D600 nm.The results showed that the best conditions for mannanase secretion were IPTG at 0.01 mmol·L-1,TB medium,induction at D600 nm=0.6-0.7,37 ℃ and 8 h of induction time.The enzyme had optimum activity at pH 7.5 and 55-60 ℃.The mannanase properties of recombinant enzyme were similar to that of the wild enzyme.The activity was stable at pH 6.5-7.5 and below 55 ℃.Most ions used in this study had no effect on it,but DTT may enhance the enzyme activity." @default.
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- W1008065051 date "2009-01-01" @default.
- W1008065051 modified "2023-09-26" @default.
- W1008065051 title "Optimization of recombinant β-mannanase of Bacillus subtilis Z-2 in promoting secretion and properties of β-mannanase." @default.
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