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- W1009725853 abstract "This chapter presents a survey of antibodies directed toward modified ribonucleosides and their basic and clinical applications. Immunoassay methodology for radioimmunoassays and enzyme linked immunosorbent assays applicable to modified nucleosides are presented.. These methods are used to characterize polyclonal and monoclonal antibodies directed toward the modified nucleosides N6-[9-(β-D-d)-6-ylcarbamoyl]-L-threonine, t6A; N6-(∆2-isopentenyl)adenosine, i6A;2ʹ-O-methyl guanosine; and zeatin. Two illustrations are presented for quantitation of nucleoside levels using immunoassay technology. One is a method for quantitating levels of modified nucleosides in tRNAs from bacterial extracts, using as an example the Escherichia coli mutant miaA that is deficient in i6A derivatives. This procedure can be adapted to screening for either prokaryotic or eukaryotic cells deficient in a specific modification on transfer RNA (tRNA) or ribosomal ribonucleicacid (rRNA). Quantitation of levels of modified nucleosides in human urine is described and polyclonal antibodies directed toward three different modified nucleosides are studied using urine samples from normal subjects and patients with cancer. The nucleoside t6A shows promise for monitoring the transition to the metastatic stage for breast cancer patients." @default.
- W1009725853 created "2016-06-24" @default.
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- W1009725853 date "1990-01-01" @default.
- W1009725853 modified "2023-09-25" @default.
- W1009725853 title "Chapter 9 Immunoassays for Modified Nucleosides of Ribonucleic Acids" @default.
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- W1009725853 doi "https://doi.org/10.1016/s0301-4770(08)61475-x" @default.
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