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- W1009870900 abstract "Publisher Summary This chapter discusses the two methods of synchronous culture, which have been most widely in use for Chlorella. In the first method, the cultures are started from a homogeneous population of small young cells—called Ds cells—which are separated from less homogeneous populations by differential centrifugation. When the cells reach a mature stag—called L3 cells—after which they do not require light for further development to complete cellular division, the light is turned off. When all the cells have completed division, the light is turned on to start the second cell cycle. In the other method, called “the method of programmed light-dark regimes,” a random culture is subjected, without being fractionated beforehand into a homogeneous population, to a programmed light and dark alternation, in which the lengths of the light and dark periods are adequately chosen according to the algal species and culture conditions. The synchrony of growth and cell division is attained on repeating the light and dark regimes several times. The technical procedures and some precautions to be taken in running the synchronous cultures by the former method are described." @default.
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- W1009870900 date "1971-01-01" @default.
- W1009870900 modified "2023-10-14" @default.
- W1009870900 title "[6] Synchronous culture of Chlorella" @default.
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- W1009870900 doi "https://doi.org/10.1016/s0076-6879(71)23082-2" @default.
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