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- W100989780 abstract "Mixtures of creatinine, glucose, and threonine with the addition of a small amount, 250 μCi (0.6 mCi/mmol) of each [U-14C]-glucose, [1-14C]-glucose or [6-14C]-glucose were heated at 180°C for 30 min in an aqueous model system. The mixtures were purified and analyzed using HPLC, a scintillation counter and the Ames test. MeIQx (2-amino-3, 8-dimethyl-imidazo-[4, 5-f] quinoxaline) and 4, 8-DiMeIQx (2-amino-3, 4,8-trimethylimidazo-[4, 5-f] quinoxaline) were detected as the main radioactive mutagens. The amount of MeIQx and 4, 8-DiMeIQx produced from threonine was estimated to be 18 and 60 nmol/mmol glucose, respectively. Radioactive carbon atoms originating from glucose were also shown to be incorporated into IQx (2-amino-3-methylimidazo-[4, 5-f]-quinoxaline). The specific activity was calculated to be 0.6, 0.3, and 0.1-0.3 mCi/mmol for MeIQx, 4, 8-DiMeIQx, and IQx, respectively, for all three labeled forms of glucose. By establishing the incorporation of carbon atoms originating from glucose into the imidazoquinoxaline mutagens it was clearly demonstrated that glucose is a precursor in the formation of these food mutagens." @default.
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- W100989780 date "2005-01-01" @default.
- W100989780 modified "2023-10-15" @default.
- W100989780 title "Incorporation of 14 C-Glucose into Mutagenic Heterocyclic Amines" @default.
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- W100989780 doi "https://doi.org/10.1533/9781845698393.3.147" @default.
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