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- W1009925899 abstract "Publisher Summary This chapter describes the preparation and properties of sulfuryl adenylates. The preparation and properties of adenosine-5′-phosphosulfate and of 3′-phosphoadenosine-5′-phosphosulfate (PAPS) are presented. Small amounts of adenosine-5′-phosphosulfate (APS) may be prepared enzymatically from ATP and inorganic sulfate by using purified ATP-sulfurylase and inorganic pyrophosphatase. APS is formed by the reaction of the pyridine–SO 3 complex with AMP in aqueous bicarbonate solution. There is probably a direct reaction between the anhydride and the substituted phosphate anion with the displacement of pyridine. The method should be a general one for the preparation of phosphate–sulfate anhydrides. In common with other nucleotides, APS may be detected on paper chromatograms and paper electrophoresis patterns by its quenching of ultraviolet fluorescence. A number of other final analytical methods may be used for the determination of the amount of PP that has disappeared or the amount of ATP that has formed as a result of the first incubation. It is found that crude enzyme systems from both liver and yeast contain the activating system for the synthesis of PAPS from ATP and inorganic sulfate." @default.
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- W1009925899 date "1963-01-01" @default.
- W1009925899 modified "2023-10-16" @default.
- W1009925899 title "[105] Preparation and properties of sulfuryl adenylates" @default.
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- W1009925899 doi "https://doi.org/10.1016/0076-6879(63)06245-5" @default.
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