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• W1010381455 abstract "A palyazati program celja, hogy rekombinans technikakat, valamint a genetikai transzformacio kulonfele eljarasait alkalmazva jaromsporas gombak karotinoid bioszinteziset modositsuk, tovabba, hogy az igy letrehozott rendszerben vizsgaljuk a bioszintetikus ut lefolyasat es a produkcio merteket. Celunk volt, hogy megvizsgaljuk a b-karotinbol kiindulo asztaxantin szintezis lehetőseget Mucor circinelloidesben, valamint karotintultermelő mutansok előallitasa. Mintegy husz modosult karotinosszetetelű (asztaxantin es/vagy kantaxantin termelesre kepes), illetve karotin tultermelő M. circinelloides torzset allitottunk elő es jellemeztunk reszletesen. Azonositottuk es reszletesen jellemeztuk a Rhizomucor miehei HMG-KoA reduktaz, valamint a M. circinelloides izopentenil pirofoszfat izomeraz genjet. Nagyszamu, a karotintermeles modositasat lehetőve tevő transzformacios vektort keszitettunk, melyek a tovabbi torzsnemesitesben felhasznalhatok. 20 jaromsporas gombafaj kulonboző, vad tipusu torzseinek karotintermeleset elemeztuk, kulonboző kornyezeti feltetelek mellett. Tobb nagy karotinoid produkciora kepes, illetve uj karotinosszetetelű torzset azonositottunk, melyek a tovabbi munkakban felhasznalhatok. Uj, a kesőbbi alap- es alkalmazott kutatasokban is alkalmazhato eljarasokat, protokolokat dolgoztunk ki elsősorban a jaromsporas gombak genetikai tramszformaciojara, a karotin termeltetes korulmenyeire (tenyesztes, fermentacio) es a termeles fokozasara vonatkozoan. | The objectives of the project were the followings: development of carotene superproducing strains by means of the over-expression of the mevalonate-isoprenoid pathway; introduction of heterologous genes into Mucor circinelloides in order to achieve the production of oxygenated b-carotene derivatives; and extension of these studies to other carotene producer Zygomycetes. Isopentenyl pyrophosphate isomerase gene (McIPP) of Mucor encoding a key-enzyme of the isoprenoid pathway was cloned and analysed. For the modification of the mevalonate pathway, functional analysis of the HMG-CoA reductase gene (hmg) of Rhizomucor miehei was completed and transforming vectors were constructed. Transformation experiments with plasmids harbouring the genes: hmg; isoA (FPP synthase); carG (GGPP synthase) and McIPP, were carried out and several strains with elevated carotene production were obtained. The crtW and crtZ genes from Agrobacterium aurantiacum were placed under the control of M. circinelloides expression signals and heterologous transformations were performed on a selected M. circinelloides strain. Transformants able to produce astaxanthin, cantaxanthin and/or zeaxanthin and β-cryptoxanthin were isolated. Agrobacterium tumefaciens-mediated transformation systems allowing integrative transformation were elaborated and optimized for M. circinelloides and other related carotenoid producer species (other Mucor, Gilbertella and Backusella species)." @default.
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• W1010381455 date "2007-01-01" @default.
• W1010381455 modified "2023-09-26" @default.
• W1010381455 title "A karotinoid termelés genetikai hátterének vizsgálata genetikailag módosított járomspórás gombák segítségével. = Molecular study of the cartenoid biosynthetic pathway using genetically modified zygomycetous fungal strains" @default.
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