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- W101044645 abstract "客观：沿着神经小径和 glial 房间的效果在 vitro 在他们的承诺的区别期间在兔子骨头髓 stromalcells (BMSC ) 调查 telomerase 活动 telomerase 的表示上的导出线的神经营养的因素(GDNF ) 。方法: BMSC 从兔子髓被获得并且把组划分了成控制， GDNF (10 ng/ml ) group.Cytokine？NSC 媒介(由我们的实验室准备了，专利没有。ZL02134314。4 ) 与 10%fetal 补充了牛的浆液(FBS ) 被用来导致 BMSC 区别神经小径。Fluorescentimmunocytochemistry 被采用识别 Nestin，神经原特定的 endase (NSE ) ，和 gial fibrillary 的表情酸的蛋白质(GFAP ) 。细胞和细胞周期的地位的生长曲线分别地被分析。在区别期间， telomerase 活动用 telomeric 重复扩大 protocol-enzyme-linked 免疫吸着剂试金(TRAP-ELISA ) 被检测。结果：BMSC 成功地被导致区分神经小径并且表示了胎儿的神经上皮，成熟神经原和 glial 房间的特定的标记。Telomeraseactivities 在沿着神经小径的区别期间在 BMSC 是无法发现的。房间生长曲线，房间周期地位和 telomerase 表示的类似的变化在二 groups.Conclusions 被观察：兔子 BMSC 不沿着神经小径在区别期间显示 telomerase 活动。GDNF 几乎不在增长和 BMSC 的 telomerase 活动上显示出很少影响。" @default.
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- W101044645 date "2006-01-01" @default.
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- W101044645 title "Lack of telomerase activity in rabbit bone marrow stromal cells during differentiation along neural pathway" @default.
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