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- W1011731330 abstract "This chapter describes a sensitive and convenient method to detect changes of oxygen in a range below 10–6 using luminous bacteria. The emission spectrum of bacterial luminescence has a broad peak around 475 nm; oxygen concentration can be calibrated by measurement of the light intensity near this wavelength using a suitable optical filter. An advantage of the use of intact luminous bacteria, and not using the isolated luciferin-luciferase system, is that otherwise the latter system requires oxygen concentrations above 10–6 M. Application of the method using the bacteria is most useful for enzyme systems for which spectrophotometric or fluorometric methods for their activity measurement can be used. Also the use of intact bacteria helps to avoid direct interaction of the oxygen indicator (the bacteria) and the oxidase system. The 1–3% NaCl and 0.5 M mannitol required for the bacteria may interfere with the enzyme system under study, and the mannitol is not harmful to mitochondria." @default.
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- W1011731330 date "1978-01-01" @default.
- W1011731330 modified "2023-09-23" @default.
- W1011731330 title "[27] Sensitive oxygen assay method by luminous bacteria" @default.
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- W1011731330 doi "https://doi.org/10.1016/s0076-6879(78)54030-5" @default.
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