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- W1014620211 abstract "This chapter discusses the purification procedure related to the enzyme Cytidine triphosphate synthetase (CTPS). CTPS was initially characterized from Escherichia coli by Lieberman and shown to catalyze the formation of cytidine triphosphate (CTP) from uridine triphosphate (UTP), adenosine triphosphate (ATP), magnesium, and ammonia. A similar reaction was later demonstrated in mammalian tissues and bacteria that used L-glutamine as an amino donor and required a guanosine nucleotide for maximum activities. The enzyme assay measures the amination of UTP spectrophotometrically at 291 nm where UTP has an extinction coefficient of 182 and CTP 1520 at pH 7.0. The assay can be carried out with either L-glutamine or ammonia serving as the amino group donor. All purification operations are performed at 0–4°C. The original purification procedure was modified to give a homogeneous protein. Purified CTP synthetase exhibits positive cooperativity for the substrates ATP and UTP." @default.
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- W1014620211 date "1978-01-01" @default.
- W1014620211 modified "2023-09-30" @default.
- W1014620211 title "[12] Cystidine triphosphate synthetase" @default.
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- W1014620211 doi "https://doi.org/10.1016/s0076-6879(78)51014-8" @default.
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