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- W1015087483 abstract "This chapter presents the techniques for the study of microtubule assembly in vitro. Microtubule assembly and disassembly in vitro is frequently monitored by turbidity (light scattering) and electron microscopy. Useful information concerning the mechanism of microtubule assembly has also been obtained with a variety of other techniques—that is, viscosity, sedimentation, flow birefringence, dark-field light microscopy, time-resolved X-ray diffraction, laser light scattering, filtration, calorimetry, and immunocytochemistry. The combination of two macromolecular techniques is often necessary, as each technique has limitations in interpretation. Turbidity measurements are useful to follow the assembly and disassembly of microtubules in vitro. Microtubules assembled in vitro are a good approximation to the long rod limit when light (320-600 nm) is used to measure turbidity. It is shown that for very long rod-like particles with a small diameter compared to the wavelength (λ) of the incident light, the turbidity is proportional to λ–3 and is a function only of the total weight concentration of scattering particles. In electron microscopt technique, the entire contents of a spray drop of solution containing microtubule protein aggregates and bushy stunt virus particles was dried on a grid after it was mixed with uranyl acetate using a dual nebulizer. The number of virus particles and the total length of microtubules or number of rings was calculated in the volume of the drop. The mass of tubulin in the various forms was determined from the concentration of virus particles, the molecular weight of the rings, and the mass per unit length of microtubules." @default.
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- W1015087483 date "1982-01-01" @default.
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- W1015087483 title "[41] Techniques for the study of microtubule assembly in vitro" @default.
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- W1015087483 doi "https://doi.org/10.1016/0076-6879(82)85043-x" @default.
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