Matches in SemOpenAlex for { <https://semopenalex.org/work/W1015598414> ?p ?o ?g. }
- W1015598414 endingPage "147" @default.
- W1015598414 startingPage "117" @default.
- W1015598414 abstract "The partition affinity ligand assay (PALA) method is normally used as a nonequilibrium method, that is, the binding reaction involved does not have to reach equilibrium. A requirement for a binding assay in aqueous two-phase systems is that the reactants in the binding reaction must have markedly different partition coefficients. The PALA principle includes incubation of the reaction mixture and subsequent separation with an added phase system, both steps being carried out in a single test tube. No separate washing steps and centrifugations are needed. In a direct binding assay, the binding between two different molecules is utilized to quantify one of them. Sufficiently concentrated aqueous solutions of some water-soluble polymers, forming phase systems, can be used for selective distribution of macromolecules. Such phase systems have a top phase rich in one of the polymers and a bottom phase rich in the other. Compared to the phase systems generally used in chemistry, that is, those containing organic solvents, such water-rich systems have been shown to be better for separating biological material." @default.
- W1015598414 created "2016-06-24" @default.
- W1015598414 creator A5008730339 @default.
- W1015598414 creator A5041402257 @default.
- W1015598414 creator A5049683340 @default.
- W1015598414 date "1982-01-01" @default.
- W1015598414 modified "2023-09-24" @default.
- W1015598414 title "Partition Affinity Ligand Assay (PALA): Applications in the Analysis of Haptens, Macromolecules, and Cells" @default.
- W1015598414 cites W1485274941 @default.
- W1015598414 cites W1497854445 @default.
- W1015598414 cites W1520632324 @default.
- W1015598414 cites W1558045689 @default.
- W1015598414 cites W1845857371 @default.
- W1015598414 cites W1965386178 @default.
- W1015598414 cites W1968045004 @default.
- W1015598414 cites W1980336289 @default.
- W1015598414 cites W1989311457 @default.
- W1015598414 cites W1992772016 @default.
- W1015598414 cites W1993625832 @default.
- W1015598414 cites W1995160428 @default.
- W1015598414 cites W1997349936 @default.
- W1015598414 cites W1998892446 @default.
- W1015598414 cites W2004017354 @default.
- W1015598414 cites W2011154070 @default.
- W1015598414 cites W2012899370 @default.
- W1015598414 cites W2016752836 @default.
- W1015598414 cites W2025724800 @default.
- W1015598414 cites W2027163663 @default.
- W1015598414 cites W2034596118 @default.
- W1015598414 cites W2035124624 @default.
- W1015598414 cites W2038871913 @default.
- W1015598414 cites W2050951459 @default.
- W1015598414 cites W2057190509 @default.
- W1015598414 cites W2060901453 @default.
- W1015598414 cites W2066496254 @default.
- W1015598414 cites W2075224514 @default.
- W1015598414 cites W2091984669 @default.
- W1015598414 cites W2093650996 @default.
- W1015598414 cites W2132465529 @default.
- W1015598414 cites W2281886671 @default.
- W1015598414 cites W243385536 @default.
- W1015598414 cites W2802764228 @default.
- W1015598414 cites W3135625888 @default.
- W1015598414 cites W565936414 @default.
- W1015598414 cites W653374124 @default.
- W1015598414 doi "https://doi.org/10.1016/s0065-2164(08)70234-x" @default.
- W1015598414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6765016" @default.
- W1015598414 hasPublicationYear "1982" @default.
- W1015598414 type Work @default.
- W1015598414 sameAs 1015598414 @default.
- W1015598414 citedByCount "7" @default.
- W1015598414 crossrefType "book-chapter" @default.
- W1015598414 hasAuthorship W1015598414A5008730339 @default.
- W1015598414 hasAuthorship W1015598414A5041402257 @default.
- W1015598414 hasAuthorship W1015598414A5049683340 @default.
- W1015598414 hasConcept C114614502 @default.
- W1015598414 hasConcept C116569031 @default.
- W1015598414 hasConcept C147483822 @default.
- W1015598414 hasConcept C149556010 @default.
- W1015598414 hasConcept C170493617 @default.
- W1015598414 hasConcept C178790620 @default.
- W1015598414 hasConcept C184651966 @default.
- W1015598414 hasConcept C185592680 @default.
- W1015598414 hasConcept C192552737 @default.
- W1015598414 hasConcept C204389451 @default.
- W1015598414 hasConcept C21951064 @default.
- W1015598414 hasConcept C29144244 @default.
- W1015598414 hasConcept C33923547 @default.
- W1015598414 hasConcept C42812 @default.
- W1015598414 hasConcept C43617362 @default.
- W1015598414 hasConcept C44280652 @default.
- W1015598414 hasConcept C521977710 @default.
- W1015598414 hasConcept C54355233 @default.
- W1015598414 hasConcept C55493867 @default.
- W1015598414 hasConcept C85196483 @default.
- W1015598414 hasConcept C86803240 @default.
- W1015598414 hasConceptScore W1015598414C114614502 @default.
- W1015598414 hasConceptScore W1015598414C116569031 @default.
- W1015598414 hasConceptScore W1015598414C147483822 @default.
- W1015598414 hasConceptScore W1015598414C149556010 @default.
- W1015598414 hasConceptScore W1015598414C170493617 @default.
- W1015598414 hasConceptScore W1015598414C178790620 @default.
- W1015598414 hasConceptScore W1015598414C184651966 @default.
- W1015598414 hasConceptScore W1015598414C185592680 @default.
- W1015598414 hasConceptScore W1015598414C192552737 @default.
- W1015598414 hasConceptScore W1015598414C204389451 @default.
- W1015598414 hasConceptScore W1015598414C21951064 @default.
- W1015598414 hasConceptScore W1015598414C29144244 @default.
- W1015598414 hasConceptScore W1015598414C33923547 @default.
- W1015598414 hasConceptScore W1015598414C42812 @default.
- W1015598414 hasConceptScore W1015598414C43617362 @default.
- W1015598414 hasConceptScore W1015598414C44280652 @default.
- W1015598414 hasConceptScore W1015598414C521977710 @default.
- W1015598414 hasConceptScore W1015598414C54355233 @default.
- W1015598414 hasConceptScore W1015598414C55493867 @default.
- W1015598414 hasConceptScore W1015598414C85196483 @default.
- W1015598414 hasConceptScore W1015598414C86803240 @default.
- W1015598414 hasLocation W10155984141 @default.