Matches in SemOpenAlex for { <https://semopenalex.org/work/W1015700512> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1015700512 endingPage "41" @default.
- W1015700512 startingPage "37" @default.
- W1015700512 abstract "For the coupled assay at 340 nm of transketolase with α-glycerophosphate dehydrogenase and triosephosphate isomerase, or with glyceraldehydes phosphate dehydrogenase, as auxiliary enzymes, a mixture of D-ribose 5-phosphate and D-xylulose 5-phosphate is needed. A mixture of D-ribose 5-phosphate and D-ribulose 5-phosphate is employed for the assay of D-ribulose-5-phosphate 3-epimerase by coupling it to the transketolase reaction. A simple procedure is described in the chapter for preparing mixtures of the pentose phosphates for the assay of the above two enzymes. Although the commercial purifed epimerase is specified, it need not be free of the isomerase, and a crude concentrate of the two enzymes from spleen, muscle, or liver, may be used provided that transketolase is absent. The ultrafiltration is carried out, at or near, 37°, to prevent the composition of the equilibrium mixture changing after the removal from the spectrophotometer. The procedure may also be used with a 20 mM solution of ribose 5-phosphate. At this higher concentration, however, further spectral changes of the isomerase product occur, and may take place before the epimerase is added, obscuring the progress of the enzymic reaction. Ribose 5-phosphate is measured, without interference from the ketopentose phosphates, by the colorimetric phloroglucinol method. Ribulose 5-phosphate is measured in ribose 5-phosphate-ribulose 5-phosphate mixtures with cysteine-carbazole; a heating time of 2 hr at 37° is used to develop the color. Xylulose 5-phosphate is measured, either alone or in conjunction with ribulose 5-phosphate by the spectrophotometric method described in the chapter. Spectrophotometric determination of D-Xylulose 5-Phosphate and D-Ribulose 5-Phosphate uses α-glycerophosphate dehydrogenase and triose phosphate isomerase to measure the glyceraldehyde 3-phosphate formed. One molecule of ketopentose phosphate causes the oxidation of 1 molecule of NADH." @default.
- W1015700512 created "2016-06-24" @default.
- W1015700512 creator A5060260440 @default.
- W1015700512 date "1975-01-01" @default.
- W1015700512 modified "2023-09-26" @default.
- W1015700512 title "[8] Preparation and analysis of mixtures of d-ribose 5-phosphate, d-ribulose 5-phosphate, and d-xylulose 5-phosphate" @default.
- W1015700512 cites W138927907 @default.
- W1015700512 cites W1507389537 @default.
- W1015700512 cites W1573203666 @default.
- W1015700512 cites W180715045 @default.
- W1015700512 cites W1968490904 @default.
- W1015700512 cites W1986514995 @default.
- W1015700512 cites W1990846267 @default.
- W1015700512 cites W2011103685 @default.
- W1015700512 cites W2031437922 @default.
- W1015700512 cites W2049216461 @default.
- W1015700512 cites W2071569479 @default.
- W1015700512 cites W2406040791 @default.
- W1015700512 doi "https://doi.org/10.1016/s0076-6879(75)41010-2" @default.
- W1015700512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1092962" @default.
- W1015700512 hasPublicationYear "1975" @default.
- W1015700512 type Work @default.
- W1015700512 sameAs 1015700512 @default.
- W1015700512 citedByCount "6" @default.
- W1015700512 crossrefType "book-chapter" @default.
- W1015700512 hasAuthorship W1015700512A5060260440 @default.
- W1015700512 hasConcept C103432212 @default.
- W1015700512 hasConcept C181199279 @default.
- W1015700512 hasConcept C185592680 @default.
- W1015700512 hasConcept C20251656 @default.
- W1015700512 hasConcept C2776317432 @default.
- W1015700512 hasConcept C2777132085 @default.
- W1015700512 hasConcept C2778727959 @default.
- W1015700512 hasConcept C2779461342 @default.
- W1015700512 hasConcept C2779966244 @default.
- W1015700512 hasConcept C2781264208 @default.
- W1015700512 hasConcept C3409486 @default.
- W1015700512 hasConcept C38706188 @default.
- W1015700512 hasConcept C43617362 @default.
- W1015700512 hasConcept C50212798 @default.
- W1015700512 hasConcept C55493867 @default.
- W1015700512 hasConceptScore W1015700512C103432212 @default.
- W1015700512 hasConceptScore W1015700512C181199279 @default.
- W1015700512 hasConceptScore W1015700512C185592680 @default.
- W1015700512 hasConceptScore W1015700512C20251656 @default.
- W1015700512 hasConceptScore W1015700512C2776317432 @default.
- W1015700512 hasConceptScore W1015700512C2777132085 @default.
- W1015700512 hasConceptScore W1015700512C2778727959 @default.
- W1015700512 hasConceptScore W1015700512C2779461342 @default.
- W1015700512 hasConceptScore W1015700512C2779966244 @default.
- W1015700512 hasConceptScore W1015700512C2781264208 @default.
- W1015700512 hasConceptScore W1015700512C3409486 @default.
- W1015700512 hasConceptScore W1015700512C38706188 @default.
- W1015700512 hasConceptScore W1015700512C43617362 @default.
- W1015700512 hasConceptScore W1015700512C50212798 @default.
- W1015700512 hasConceptScore W1015700512C55493867 @default.
- W1015700512 hasLocation W10157005121 @default.
- W1015700512 hasLocation W10157005122 @default.
- W1015700512 hasOpenAccess W1015700512 @default.
- W1015700512 hasPrimaryLocation W10157005121 @default.
- W1015700512 hasRelatedWork W1500576496 @default.
- W1015700512 hasRelatedWork W1549263665 @default.
- W1015700512 hasRelatedWork W1599787098 @default.
- W1015700512 hasRelatedWork W190286435 @default.
- W1015700512 hasRelatedWork W1985334975 @default.
- W1015700512 hasRelatedWork W1989619804 @default.
- W1015700512 hasRelatedWork W2028995352 @default.
- W1015700512 hasRelatedWork W2062873229 @default.
- W1015700512 hasRelatedWork W2253556859 @default.
- W1015700512 hasRelatedWork W4255798301 @default.
- W1015700512 isParatext "false" @default.
- W1015700512 isRetracted "false" @default.
- W1015700512 magId "1015700512" @default.
- W1015700512 workType "book-chapter" @default.