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- W101572434 abstract "Research Article15 September 1994free access Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. J.D. Brown J.D. Brown Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author B.C. Hann B.C. Hann Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author K.F. Medzihradszky K.F. Medzihradszky Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author M. Niwa M. Niwa Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author A.L. Burlingame A.L. Burlingame Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author P. Walter P. Walter Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author J.D. Brown J.D. Brown Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author B.C. Hann B.C. Hann Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author K.F. Medzihradszky K.F. Medzihradszky Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author M. Niwa M. Niwa Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author A.L. Burlingame A.L. Burlingame Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author P. Walter P. Walter Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. Search for more papers by this author Author Information J.D. Brown1, B.C. Hann1, K.F. Medzihradszky1, M. Niwa1, A.L. Burlingame1 and P. Walter1 1Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448. The EMBO Journal (1994)13:4390-4400https://doi.org/10.1002/j.1460-2075.1994.tb06759.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The signal recognition particle (SRP) is an evolutionarily conserved ribonucleoprotein (RNP) complex that functions in protein targeting to the endoplasmic reticulum (ER) membrane. Only two protein subunits of the SRP, Srp54p and Sec65p, and the RNA subunit, scR1, were previously known in the yeast Saccharomyces cerevisiae. Purification of yeast SRP by immunoaffinity chromatography revealed five additional proteins. Amino acid sequencing and cloning of the genes encoding four of these proteins demonstrated that the yeast SRP contains homologs (termed Srp14p, Srp68p and Srp72p) of the SRP14, SRP68 and SRP72 subunits found in mammalian SRP. The yeast SRP also contains a 21 kDa protein (termed Srp21p) that is not homologous to any protein in mammalian SRP. An additional 7 kDa protein may correspond to the mammalian SRP9. Disruption of any one of the four genes encoding the newly identified SRP proteins results in slow cell growth and inefficient protein translocation across the ER membrane. These phenotypes are indistinguishable from those resulting from the disruption of genes encoding SRP components identified previously. These data indicate that a lack of any of the analyzed SRP components results in loss of SRP function. ScR1 RNA and SRP proteins are at reduced levels in cells lacking any one of the newly identified proteins. In contrast, SRP components are present at near wild type levels and SRP subparticles are present in cells lacking either Srp54p or Sec65p. Thus Srp14p, Srp21p, Srp68p and Srp72p, but not Sec65p or Srp54p, are required for stable expression of the yeast SRP. Previous ArticleNext Article Volume 13Issue 181 September 1994In this issue RelatedDetailsLoading ..." @default.
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