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- W1015920004 abstract "This chapter discusses cells and subcellular particles. Most of the chromatographic separations of cells can be considered as adherence chromatography—for example, a specified type of cell is more or less selectively adsorbed on the bed used and eluted after the removal of contaminating substances. However, the second step is frequently omitted and replaced with a batch procedure, during which the column bed is destroyed and adhering cells are extracted from the material and removed from the column. In general, the chromatographic procedures used for cells and subcellular particles are used for strictly preparative purposes. It is recommended that the column should be equilibrated by pumping several column volumes of water through the bed. If column backpressure increases, which happens frequently shortly after the column has been set in operation, it is advised that the direction of flow of the water through the column should be reversed. Alternatively, it is also possible to fill the column with dry porous glass and then to pump water through the bed." @default.
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- W1015920004 date "1975-01-01" @default.
- W1015920004 modified "2023-09-25" @default.
- W1015920004 title "Chapter 50 Cells and subcellular particles" @default.
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- W1015920004 doi "https://doi.org/10.1016/s0301-4770(08)61214-2" @default.
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